Journal: Frontiers in Physiology
Article Title: Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator
doi: 10.3389/fphys.2020.00345
Figure Lengend Snippet: cLSM images of control and DSS rat cardiomyocytes before and after sGC activator treatment demonstrating sGC translocation to the intercalated disc (A–D) . Representative immunofluorescence images of cardiomyocytes stained for cardiac sGC (red) and counterstained for α-actinin (green). (E–H) Immunofluorescence images of cardiomyocytes stained for connexin 43 (red) and counterstained for α-actinin (green). (I–L) Representative immunofluorecence images of cardiomyocytes stained for connexin 43 (green) and sGC (red).
Article Snippet: Immuno-stained samples were analyzed by confocal laser scanning microscopy (cLSM) (Nikon Eclipse Ti-E Inverted Microscope System; Nikon Instruments, Nikon Corp, Shinagawa, Tokyo, Japan).
Techniques: Control, Translocation Assay, Immunofluorescence, Staining